NIAGADS
  • VCPA
  • Introduction
  • Step 1: Set up the Amazon Web Services (AWS) environment
    • 1.1 Create AWS account
    • 1.2 Configure your computing environment and login to AWS
    • 1.3 Setup a S3 bucket (simple storage solution for AWS) for hosting sequencing data
    • 1.4 Install AWS command line software for accessing S3 bucket via command line interface
    • 1.5 Install StarCluster for AWS instance provisioning (optional)
  • Step 2: Create your tracking database instance
    • Option 1: Setup sample tracking database using Public AMI (recommended)
    • Option 2: Setup sample tracking database using Docker
  • Step 3: Configure your project information in the tracking database
    • 3.1 List all projects in the tracking database
    • 3.2 Create the project in the tracking database
  • Step 4: Upload sequencing data to your S3 bucket
  • Step 5: Configure your samples information in the tracking database
    • 5.1 Input the sample information to the tracking database
    • 5.2 Populate the tracking database with the S3 paths for the samples to be processed
    • 5.3 Populate the tracking database with the designated result folder for each sample to be processed
    • 5.4 Input PCR protocol information into the tracking database
    • 5.5 Add the capture kit information (WES sample only) into the tracking database
    • 5.6 Generate an ID to represent the capture kit information (WES sample only)
  • Step 6: Submit a job to process one whole genome (WGS) / whole exome (WES) sample
    • 6.1 Update vcpa-pipeline bitbucket contents
    • 6.2 Choose which workflow to use
    • 6.3 Enter your AWS credentials into the workflow script
    • 6.4 Launch Amazon EC2 Spot Instances via starcluster
  • Step 7: Review quality metrics of processed data
  • Step 8: Generating Project-level VCF via joint genotyping
  • Optional: Change software versions and dependencies of the VCPA workflow
Powered by GitBook
On this page
  1. Step 5: Configure your samples information in the tracking database

5.4 Input PCR protocol information into the tracking database

VCPA will process the sequencing data differently, dependent on whether they are generated using the PCR free or non-PCR free protocol. Users will therefore need to input this information into the "isPCR_free" column of the tracking database.

Users can type the following command:

curl -sS "http://IP/v1/sample/set-attr/isPCR_free/${project_id}/${sample_name}/${0|1}"

GET http://IP/v1/sample/set-attr/isPCR_free/${project_id}/${sample_name}/${0|1}

Add is the value of PCR free or non-PCR free.

Path Parameters

Name
Type
Description

project_id

string

This is the project ID outputted by Section 3.2.

sample_name

string

Sample name information.

value

boolean

Enters 1 if the sample is generated using a "PCR free protocol", otherwise, type "0" here.

{
    "status":"success",
    "id":"4"
}

Previous5.3 Populate the tracking database with the designated result folder for each sample to be processedNext5.5 Add the capture kit information (WES sample only) into the tracking database

Last updated 6 years ago